The book provides a historical overview of the principles and development of vitrification techniques in animal and humans, the various consumables utilized for the vitrification of cells and tissue and suppliers thereof; the vitrification and warming technique for oocytes, sperm, pronuclear stage embryos, cleavage stage embryos, blastocysts, ovarian tissue and embryonic stem cells. Wherever applicable, the setting up including protocol, operation, maintenance and trouble-shooting of the different protocols used for vitrifying various stages of cells and tissues is also covered.