The book focuses on supercritical fluids as a green alternative reaction medium in catalytic reactions involving lipases. Lipases have great potential in industrial applications and the book describes their main sources, reaction kinetics, structure, physicochemical characteristics, and applications. Techniques of isolating, extracting and purifying are given as are lipase immobilization and its advantages over soluble lipases, immobilization techniques, immobilization materials, and bioreactor configurations using immobilized lipase. The advantages of non-aqueous media in biochemical synthesis are discussed. The book includes a case study on using SC-CO2 for biodiesel production