This cutting-edge reference book compiles standard operating procedures, protocols, and applications of Next-Generation Sequencing (NGS). It discusses genomic testing applications through NGS. It pays special focus on the protocols for cataloging variants of uncertain significance. Over the years, NGS and advanced bioinformatics approaches have allowed the transition of genomic assays into translational practices. The book covers visualization of NGS data sets, investigation of early development impairment, and metagenome protocols. It also discusses the challenges in NGS methods. Key Points-· Includes case studies of application of next-generation sequencing in different taxa like humans, rodents, plants, bacteria· Compiles protocols from various reputed companies like Illumina, PacBio, ThermoFisher· Discusses the translational applications of NGS methods· Reviews Machine Learning Heuristics For NGS data interpretation· Discusses emerging genomic assay technologies and characterizing mechanisms of disease prevalence. The book is meant for researchers and industry experts in genomics, computational biology, and bioinformatics.