Gnotobiotic Mouse Technology: An Illustrated Guide is a manual for the maintenance, husbandry, and experimental manipulation of germ free and gnotobiotic mice. Its goal is to assist investigators in establishing and successfully maintaining a facility for germ free mouse research. The techniques and procedures covered are broken down into modules, each of which contains a step-bystep, practical, and visual guide to a set of related procedures. Each description consists of a materials list, introduction, and general overview, followed by a complete illustrated description of all the steps for each procedure. Following each description, troubleshooting tips and hints are described.