This laboratory manual comprehensively reviews the essential laboratory practices and different biochemistry protocols. The initial chapters of the book provide an overview of lab safety protocols, focusing on the importance of accuracy and precision in experimental procedures. It covers essential topics such as laboratory setup, proper handling and maintenance of lab apparatus, and waste disposal. It provides a detailed exploration of spectrophotometry principles and assays, along comprehensive cell biology techniques, including staining and microscopy. The book also addresses qualitative and quantitative analyses of carbohydrates, amino acids, proteins, and lipids, providing methods for extraction and characterization. The book further details the extraction, purification, and characterization of enzymes and presents enzymatic assays and studies on enzyme kinetics, providing a comprehensive understanding of enzyme activity and regulation. The final section introduces hematology techniques, including blood smear preparation and various blood parameter determinations. It also covers forensic tests for blood detection and serum protein electrophoresis. This book is useful for graduate and post graduate students of biochemistry, molecular biology, and microbiology.