"This book aims to deliver to the reader updated information about the thematic biotechnique of cryopreservation of germ cells and gonads of South America Neotropical fish. The protocols development of cryopreservation for South American fish have been developed since the beginning approximately 40 years ago, as the concepts, equipment, techniques and analyses for the validation of the results also improved. The chapters' were planned with the objective of a narrative that shows to the reader the need and importance of a fish germplasm bank development, either for a commercial production or ichthyofauna conservation. Although the aim of the biobank use does not change in relation to its technical structure (semen diluted in a cryoprotectant solution associated to extender(s)), the practical application conceptually evolved"-- Provided by publisher.