This book provides an overview of botanical extract development, its standardization, advanced analytical technologies, and innovative applications in pharmaceuticals, nutraceuticals, and sustainable agriculture. The chapters introduce the historical evolution of botanical extracts and the fundamental roles of secondary metabolites. It further explores traditional and modern extraction techniques, the impact of extraction methods on extract efficacy, and advanced analytical technologies including HPLC, mass spectrometry, and AI-driven approaches for quality control. Furthermore, Botanical Extracts: Research Methods and Applications in Pharmaceutical Sciences presents biotechnological and nanotechnological advances in extraction and delivery systems, discusses the pharmacokinetics and pharmacodynamics of botanical compounds, and highlights challenges such as adulteration and supply chain variability. Toward the end, the book addresses emerging applications of extract in eco-friendly agriculture, as well as marine botanical extracts, innovative formulation strategies, and future trends in botanical research and development.