This book provides an in-depth exploration of siderophores and related natural products, focusing on their biosynthesis, enzymology, and applications. It is part of the Methods in Enzymology series and includes detailed methodologies for the study of siderophore production, structure, and function. The book is edited by Timothy Wencewicz and includes contributions from experts in the field, offering comprehensive insights into the molecular biology and chemistry of siderophores. It is designed for researchers and professionals in biochemistry, molecular biology, and related fields, aiming to advance understanding of siderophore-mediated processes and their potential applications in medicine and biotechnology.